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Optical microscopy: the resolution revolution
  Öppna föreläsningar på Albanova

Friday 20 November 2015
from 09:15 to 11:00
at Oskar Klein / Svedberg Hall
Speaker : Stefan W Hell (Max Planck Institute for Biophysical Chemistry, Göttingen, Germany)
Abstract :

Stefan W Hell, 2014 year Nobel prize winner in Chemistry and the inventor and pioneer of super-resolution microscopy, will become honorary doctor at KTH. In conjunction to this event, on Friday the 20th of November, he will give a lecture entitled "Optical microscopy: the resolution revolution". The lecture will be followed by a panel discussion, where there will be opportunities to ask questions and discuss super-resolution microscopy and science in general with Stefan Hell.

Time: 20/11, 9:15-10:00 (Open lecture), 10:15-11:00 (Panel discussion)

Place: Albanova University Center, Oskar Klein Auditorium (open lecture), Svedberg Hall (panel discussion, located next to Oskar Klein)

Abstract: Throughout the 20th century it was widely accepted that a light microscope relying on conventional optical lenses cannot discern details that are much finer than about half the wavelength of light (200-400 nm), due to diffraction. However, in the 1990s, the viability to overcome the diffraction barrier was realized and microscopy concepts defined, that can resolve fluorescent features down to molecular dimensions. In this lecture, I will discuss the simple yet powerful principles that allow neutralizing the limiting role of diffraction1,2. In a nutshell, feature molecules residing closer than the diffraction barrier are transferred to different (quantum) states, usually a bright fluorescent state and a dark state, so that they become discernible for a brief period of detection. Thus, the resolution-limiting role of diffraction is overcome, and the interior of transparent samples, such as living cells and tissues, can be imaged at the nanoscale.


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