Investigation of biomolecules using mass spectrometry based methods
Molecular Physics seminar
Monday 20 October 2014
to 11:00 at
Oleksii Rebrov (Molecular Physics Department)
Mass spectrometry (MS) has become a powerful tool of investigation in many
fields of studies such as molecular physics, analytical chemistry and
proteomics. The fast and sensitive performance of MS places it among the leading
techniques in drug analysis and development. Furthermore, MS represents a promising
approach to investigate structure and properties of biomolecules.
Commonly used fragmentation techniques for peptide sequencing and recognition
cannot provide fragmentation patterns for full characterization of
selected precursor. They usually complement each other providing
additional information about investigated polypeptide. The
possibility of activating electrospray ionization produced polypeptide ions using a
high-energy beam of positively charged air ions was investigated at the
Physiological chemistry division, Karolinska Institutet. Obtained results will be described in this talk.
The second part of presentation concerns the recent results of the project focused on the
investigation of fundamental principles of chiral recognition and selection. Although
tandem mass spectrometry has proven to be a highly promising method of
quantitative chiral analysis, almost nothing is known concerning
fundamental physical processes which lie behind gas-phase chiral
recognition via activated fragmentation.
Collisions activated dissociation of L- and D-forms of protonated amino acids with
non-chiral and chiral targets were studied using MS based method on the
ion source platform at the Department of Physics, Stockholm University.